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Research groups from Fujian Institute of Research on the Structure of Matter have made progress on the dissolution-enhanced luminescent bioassay (DELBA) Based on Inorganic Lanthanide Nanoparticles.

Source:Rare earth of China Date:2015-06-01

    The research groups headed by Prof. CHEN Xueyuan, at Key Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences and Prof. HUANG Mingdong, at State Key Laboratory of Structural Chemistry and Danish-Chinese Centre have recently developed a unique and ultrasensitive bioassay method, i.e. dissolution-enhanced luminescent bioassay (DELBA), based on the dissolution-enhanced PL of inorganic Ln3+-NPs.

    By simply replacing Ln3+-chelates with Ln3+ NPs in conventional DELFIA protocol, the proposed DELBA can effectively enhance the labelling ratio of Ln3+ per biomolecule, which greatly improves the PL signal (106 times) and detection sensitivity. By employing sub-10 nm NaEuF4 NPs along with enhancer solution containing β-NTA, the group revealed the mechanism of the dissolution-enhanced PL of the NPs. Utilizing the biotinylated NaEuF4 NPs as dissolution-enhanced luminescent Nano probes, they demonstrated the ultrasensitive and accurate detection of an important tumor marker CEA in human serum samples with a limit of detection (LOD) down to 0.1 pg/ml (0.5 FM), which is the lowest among those ever reported (about three orders of magnitude improvement relative to that of DELFIA).  The CEA levels (for 20 human serum samples from a tumour hospital) derived from DELBA were compared with those independently measured using a commercial DELFIA kit, and a good agreement was found between both methods. Further indicators such as the coefficient of variations and the recoveries of the assays revealed the excellent accuracy and precision of the proposed DELBA. Results of this study have been published as a communication in Angew. Chem. Int. Ed. (Angew. Chem. Int. Ed. 2014, 53, DOI: 10.1002/anie.201405937) on 11st Aug., 2014 and filed for Chinese and International patent application under the PCT. 

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